Mechanism of endocytic regulation of intestinal tight junction remodeling during nutrient starvation in jejunal IPEC?J2 cells

Intestinal epithelial cells are tightly bound by tight junction proteins (TJP) which dynamic and sensitive to environmental stress. However, the role of endocytic pathway in regulation TJP abundance integrity during nutrient stress is poorly understood. Therefore, this study was conducted investigate starvation mechanism process. IPEC-J2 were subjected Krebs-Ringer bicarbonate buffer (KRB) TJP, an indication remodeling, characterized with RT-PCR, western blotting immunofluorescence. Abundance dynamically regulated starvation. The protein levels claudin-1, 3, 4 initially downregulated within first 6 hours starvation, then, increased thereafter (P < .01). there no change occludin ZO-1. Lysosome proteasome inhibitors used determine contribution these degradation pathways remodeling. Short-term starvation-induced found be lysosome dependent. Specifically, downregulation claudin-3 via a dynamin-dependent, but clathrin caveolae independent, partly reversed amino acids supplementation. Interestingly, re-synthesis prolonged depended on function. Collectively, study, for time, elucidated major dynamin-dependent endocytosis intestinal cells. transient inhibition may potential preserving function metabolic or pathological states such as inflammatory bowel disease that involves destruction TJP.